Embryonic Fibroblast-Conditioned Medium Enhances Viability and Proliferation of Chick Circulating Primordial Germ Cells (cPGCs) in Suspension Culture.

Review of Differentiation and Proliferation of Primordial Germ Cells in Culture

Primordial germ cells (PGCs) are highly specialized cell population that arises from the epiblast in vivo. There are three critical steps in the life cycle of these cells: 1-Specification 2-migration and proliferation 3-prenatal and postnatal sex specific development. Specification of germ cells in epiblast occurs due to signals secreted from extraembryonic tissues. Primordial germ cells are re...

The effect of BMP4 on mouse embryonic stem cell proliferation and differentiation into primordial germ cells

Background and Aim: Artificial gamete production from stem cells is a novel strategy for treatment of infertility. Among various stem cell sources, embryonic stem cells (ESC) can be considered as an appropriate source for in vitro formation of germ cells. In this study we evaluated the effect of BMP4 on proliferation and differentiation of mouse embryonic stem cells into primordial germ cells (...

Isolation and characterisation of chick embryonic primordial germ cells

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Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

Proliferation in culture of primordial germ cells derived from embryonic stem cell: induction by retinoic acid

An in vitro system that supports primordial germ cells (PGCs) survival and proliferation is useful for enhancement of these cells and efficient transplantation in infertility disorders. One approach is cultivation of PGCs under proper conditions that allow self-renewal and proliferation of PGCs. For this purpose, we compared the effects of different concentrations of retinoic acid (RA), and the...